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Automatic in vivo microscopy video mining for leukocytes
2007
SIGKDD Explorations
Automatic spatiotemporal mining of moving cells from in vivo microscopy videos is extremely difficult because of the severe noises, camera/subject movements, deformations, and strong dependencies on microscopy ...
Biological videos are very different from conventional videos. ...
Several approaches have been proposed for tracking moving leukocytes from in vivo microscopy video sequences. ...
doi:10.1145/1294301.1294309
fatcat:uktquem36bduvcfiio56aa5otm
An automated method for analysis of flow characteristics of circulating particles from in vivo video microscopy
2005
IEEE Transactions on Medical Imaging
The task of automated blood cell tracking in vessels from in vivo video is particularly challenging because of the blood cells' nonrigid shapes, the instability inherent in in vivo videos, the abundance ...
All these results suggest that our method can in fact constitute a reliable replacement for manual extraction of blood flow characteristics from in vivo videos. ...
Examples of two frames taken from two different in vivo video microscopy of rat mesenteric microvasculature are shown in Fig. 11(a), (b) . ...
doi:10.1109/tmi.2005.851759
pmid:16092333
fatcat:s57ayf7oujhvhkw7xpupgjlrri
A Monte Carlo approach to rolling leukocyte tracking in vivo
2006
Medical Image Analysis
Tracking the movement of rolling leukocytes in vivo contributes to the understanding of the mechanism of the inflammatory process and to the development of anti-inflammatory drugs. ...
From tracking within 99 intravital microscopic video sequences, the Monte Carlo tracker exhibits superior performance in the reduced localization error and the increased number of frames tracked when compared ...
In this paper, we develop a Monte Carlo tracker to track a single rolling leukocyte in vivo. ...
doi:10.1016/j.media.2006.05.006
pmid:16876461
fatcat:76r3uu3l7vdmxowbk6mfq6dipq
Detection of leukocytes in contact with the vessel wall from in vivo microscope recordings using a neural network
2000
IEEE Transactions on Biomedical Engineering
Sequences of video images are obtained and digitized with a frame grabber. A method for automatic detection and characterization of leukocytes in the video images is developed. ...
The leukocyte-vessel wall interactions are studied in post capillary vessels by intravital video microscopy during in vivo animal experiments. ...
It is our objective to develop an approach for detection and tracking of individual leukocytes in sequences of video images from in vivo microcirculation experiments. ...
doi:10.1109/10.846689
pmid:10916266
fatcat:p4bjkpwuqvc3vefflx7ilzeznu
Optical Flow Based Analysis of Leukocyte Dynamics in Microcirculation
2015
International Journal of Artificial Intelligence & Applications
In this paper we adopt a duality based TV-L1 optical flow to analyze leukocyte behavior using in vivo video microscopy. ...
Using this technique, it is possible to directly compute the trajectories of leukocytes and their physical interaction parameters directly from the video frames. ...
Figure1: Ten successive typical frames of rolling and adherent leukocytes from in vivo color video clip Figure 1 shows typical frames of rolling and adherent leukocytes from in vivo color video clip. ...
doi:10.5121/ijaia.2015.6303
fatcat:6lcdajbyffh7pjruxgaypszk7i
Novel Molecular Imaging Approach for Subclinical Detection of Iritis and Evaluation of Therapeutic Success
2010
American Journal of Pathology
In vivo MS accumulation preceded clinical signs of anterior uveitis and leukocyte adhesion in iris vasculature. ...
This work introduces noninvasive subclinical detection of endothelial injury in the iris vasculature , providing a unique opportu-nity for quantifying vascular injury and immune response in vivo. ...
Garland for help in the preparation of this manuscript. ...
doi:10.2353/ajpath.2010.100007
pmid:20581051
pmcid:PMC2893649
fatcat:4ww5zqsryjfp7epc4ggurgp4dm
Uropod elongation is a common final step in leukocyte extravasation through inflamed vessels
2012
Journal of Cell Biology
Using enhanced three-dimensional detection of fluorescent CD18 fusion proteins in a newly developed knockin mouse, we report that extravasating leukocytes (neutrophils, monocytes, and T cells) show delayed ...
Despite considerable advances in our understanding of cell migration in secondary lymphoid organs, real-time leukocyte recruitment into inflamed tissues is not well characterized. ...
This project was financially supported through grants from the National Institutes of Health (HL087088 and HL018208 to M. Kim) ...
doi:10.1083/jcb1977oia11
fatcat:nvkycqbuxvcfpn7bijivtuykka
Uropod elongation is a common final step in leukocyte extravasation through inflamed vessels
2012
Journal of Experimental Medicine
Using enhanced three-dimensional detection of fluorescent CD18 fusion proteins in a newly developed knockin mouse, we report that extravasating leukocytes (neutrophils, monocytes, and T cells) show delayed ...
Despite considerable advances in our understanding of cell migration in secondary lymphoid organs, real-time leukocyte recruitment into inflamed tissues is not well characterized. ...
This project was financially supported through grants from the National Institutes of Health (HL087088 and HL018208 to M. Kim) ...
doi:10.1084/jem.20111426
pmid:22711877
pmcid:PMC3405502
fatcat:tja744lwefgfdp4iphfankrw4i
Selective Uptake of Indocyanine Green by Reticulocytes in Circulation
2003
Investigative Ophthalmology and Visual Science
It has been suggested that ICG labels leukocytes and that ICG can be used to track leukocyte movement in vivo. ...
The strongly ICG-labeled cells did not roll on dermal vascular endothelium in vivo, in contrast to rhodamine 6G-labeled leukocytes. ...
Acknowledgments The authors thank Ulrich von Andrian for first pointing out the lack of interactions between ICG-labeled cells and the endothelial wall, and Pat P. ...
doi:10.1167/iovs.03-0041
pmid:14507897
pmcid:PMC2830078
fatcat:o5kpky2ghfdwhdw3gpeehuw37q
In Vivo Imaging of the Mouse Neurovascular Unit Under Chronic Cerebral Hypoperfusion
2014
Stroke
In the pial small arteries and veins, rolling and adhesion of leukocyte were detected, more prominently in the latter. ...
In the deep cortical capillaries, flow stagnation because of leukocyte plugging was frequently observed. Conclusions-We established an in vivo imaging method for real-time visualization of the NVU. ...
Sources of Funding This work was supported by Grants-in-Aid for Scientific Research (KAKENHI) grant number 22590929.
Disclosures None. ...
doi:10.1161/strokeaha.114.005891
pmid:25370583
fatcat:2b3fb7cnondh7juutqepubjvwe
Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence
2010
Optics Express
We describe a new method for imaging leukocytes in vivo by exciting the endogenous protein fluorescence in the ultraviolet (UV) spectral region where tryptophan is the major fluorophore. ...
Two-photon excitation near 590 nm allows noninvasive optical sectioning through the epidermal cell layers into the dermis of mouse skin, where leukocytes can be observed by video-rate microscopy to interact ...
(d) Single-frame excerpts from video recordings of leukocyte migration in skin tissue (Media 3). (e) Trajectory of the centroid of a migrating leukocyte at 80-second interval (scale bar 20 µm). ...
doi:10.1364/oe.18.000988
pmid:20173920
pmcid:PMC3369551
fatcat:4eylt6frrfbltezdoc5faze25e
Page 57 of None Vol. 38, Issue 2
[page]
1998
None
The migration of leukocytes from the blood- stream into inflamed tissue requires a cascade of events in the microcirculation (24). ...
Soluble adhesion molecules are detectable only in small quantities in serum of healthy individuals (21, 22). ...
Modeling leukocyte trafficking at the human blood-nerve barrier in vitro and in vivo geared towards targeted molecular therapies for peripheral neuroinflammation
2016
Journal of Neuroinflammation
Modeling leukocyte trafficking at the blood-nerve barrier using a reliable human in vitro model and potential intravital microscopy techniques in representative animal models guided by human observational ...
Peripheral neuroinflammation is characterized by hematogenous mononuclear leukocyte infiltration into peripheral nerves. ...
and a subaward P30 AI27767 to E.E.U., as well as institutional funds from the University of Alabama at Birmingham. ...
doi:10.1186/s12974-015-0469-3
pmid:26732309
pmcid:PMC4702318
fatcat:tdrfztnlv5g4vdqaj276u4inse
Automatic Tracking of Rolling Leukocytes in Vivo
2002
Microvascular Research
Automated tracking of leukocytes is possible for in vitro studies, but not for recordings resulting from intravital experiments. ...
Therefore, we have designed and implemented an image processing system for automated tracking of rolling leukocytes in vivo. ...
ACKNOWLEDGMENTS This work was in part supported by NIH R01 HL 64381 to KL. STA is supported in part by the Army Research Office under DAAD19-01-1-0594. ...
doi:10.1006/mvre.2001.2373
pmid:11749081
fatcat:drckvcfmbjfohb4q7lck6w4jzq
Monocytes, neutrophils, and platelets cooperate to initiate and propagate venous thrombosis in mice in vivo
2012
Journal of Experimental Medicine
Using conditional mutants and bone marrow chimeras, we show that intravascular activation of the extrinsic pathway of coagulation via tissue factor (TF) derived from myeloid leukocytes causes the extensive ...
We describe a novel mouse model of DVT which reproduces a frequent trigger and resembles the time course, histological features, and clinical presentation of DVT in humans. ...
Massberg), as well as the National Institutes of Health grant HL006350 (N. Mackman) and the National Heart, Lung, and Blood Institute of the National Institutes of Health grant HL102101 (D.D. ...
doi:10.1084/jem.20112322
pmid:22451716
pmcid:PMC3328366
fatcat:uaxrphi6nze6zmxk7caotn6cku
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