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Cytoskeletal Proteins in Caulobacter crescentus: Spatial Orchestrators of Cell Cycle Progression, Development, and Cell Shape
[chapter]
2017
Sub-cellular biochemistry
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Cell polarity of vibrioid C. crescentus cells is marked by the presence of a stalk at one end in the stationary form and a polar flagellum in the motile form. ...
Caulobacter crescentus, an aquatic Gram-negative α-proteobacterium, is dimorphic, as a result of asymmetric cell divisions that give rise to a free-swimming swarmer daughter cell and a stationary stalked ...
Research in the Goley laboratory relevant to the subject of this chapter is supported by the National Institutes of Health under award number R01GM108640 (to E.D.G). ...
doi:10.1007/978-3-319-53047-5_4
pmid:28500524
pmcid:PMC5554946
fatcat:bz26v56sjndp7ox6fb3uhucel4
A Self-Associating Protein Critical for Chromosome Attachment, Division, and Polar Organization in Caulobacter
2008
Cell
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Here, we provide evidence that Caulobacter crescentus uses a multimeric pole-organizing factor (PopZ) that serves as a hub to concurrently achieve several polarizing functions. ...
Instead, evidence suggests that localization of PopZ largely relies on PopZ multimerization in chromosome-free regions, consistent with a self-organizing mechanism. ...
We address this fundamental question in the α-proteobacterium Caulobacter crescentus whose life cycle is rich in well-documented polarized events (Lawler and Brun, 2007) . ...
doi:10.1016/j.cell.2008.07.016
pmid:18805089
pmcid:PMC2614312
fatcat:7es4cjaebrfr5llwmu62ycg6oa
Chromosome Driven Spatial Patterning of Proteins in Bacteria
2010
PLoS Computational Biology
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In the asymmetrically dividing bacteria Caulobacter crescentus, a scaffolding protein, PopZ, localizes to both poles and aids the differential patterning of proteins between mother and daughter cells during ...
Our model reproduces all the observed patterns of PopZ and misfolded protein localization -from diffuse, unipolar, and bipolar patterns and can also account for the observed patterns in a variety of mutants ...
Supporting Information Figure 1 . 1 Schematics of observed patterns and model for protein localization in bacteria. (a) Schematic of PopZ localization during cell cycle of Caulobacter crescentus. ...
doi:10.1371/journal.pcbi.1000986
pmid:21085680
pmcid:PMC2978675
fatcat:saeiy5tganfgjh5woq5h4xeecy
CaulobacterPopZ forms an intrinsically disordered hub in organizing bacterial cell poles
2016
Proceedings of the National Academy of Sciences of the United States of America
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At the cell poles of Caulobacter crescentus, a 177-amino acid (aa) protein called PopZ self-assembles into 3D polymeric superstructures. ...
Together, the N-terminal 133 aa of PopZ are sufficient for interacting with all binding partners, even in the absence of homooligomeric assembly. ...
This work was supported in part by National Science Foundation Grants MCB-1518171 (to G.R.B.) and CHE-1413696 (to K.V.), and NIH Grant P20GM103432 (to K.V.). ...
doi:10.1073/pnas.1602380113
pmid:27791060
pmcid:PMC5098656
fatcat:67ikabxlzbetjk34mldelgop2y
Bacterial scaffold directs pole-specific centromere segregation
2014
Proceedings of the National Academy of Sciences of the United States of America
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The bacterium Caulobacter crescentus uses a ParA-based partitioning system to segregate newly replicated chromosomal centromeres to opposite cell poles. ...
We identify mutations in PopZ that allow scaffold assembly but specifically abrogate interactions with ParA and demonstrate that PopZ/ParA interactions are required for proper chromosome segregation in ...
(B) Model for PopZ modulation of ParA activity in the context of the Caulobacter cell during replication and centromere segregation. ...
doi:10.1073/pnas.1405188111
pmid:24778223
pmcid:PMC4024888
fatcat:6hmd7x5tjjfvdbuycsshlanlre
The Polar Organizing Protein PopZ Is Fundamental for Proper Cell Division and Segregation of Cellular Content in Magnetospirillum gryphiswaldense
2019
mBio
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Moreover, substitution of PopZ orthologs between M. gryphiswaldense and the related alphaproteobacterium Caulobacter crescentus indicated that PopZ localization depends on host-specific cues and that both ...
Furthermore, we show that PopZ localization and function differ from other nonmagnetotactic alphaproteobacterial model organisms. ...
The help of Annika Stüven and Julian Herz during PopZ ortholog replacement experiments is greatly acknowledged. ...
doi:10.1128/mbio.02716-18
fatcat:zkmganozoneo7no4qc7tnn2aaq
Regulation of cell polarity in bacteria
2014
Journal of Cell Biology
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PopZ in Caulobacter crescentus has functional similarity to DivIVA but the two proteins are not homologues. ...
In the filament-pulling model (Gerdes et al., 2010) , ParA polymerizes into a DNA-bound filament upon ATP binding. ...
doi:10.1083/jcb.201403136
pmid:25002676
pmcid:PMC4085708
fatcat:hntvgc6denebdlzagd2u4fkat4
Oligomerization and higher-order assembly contribute to sub-cellular localization of a bacterial scaffold
2013
Molecular Microbiology
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In Caulobacter crescentus, the PopZ polar scaffold protein supports asymmetric cell division by recruiting distinct sets of binding partners to opposite cell poles. ...
Thus, PopZ undergoes multiple orders of self-assembly, and the formation of an interconnected superstructure is a key feature of polar organization in Caulobacter. ...
LRC was supported by Office of Basic Energy Sciences, Biological and Environmental Research, of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. ...
doi:10.1111/mmi.12398
pmid:24102805
pmcid:PMC3859194
fatcat:s6wo43p4gfe6tiftibpmt2am3q
Chromosome architecture is a key element of bacterial cellular organization
2012
Cellular Microbiology
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In addition, it is becoming increasingly clear that yet another level of information is encoded by the bacterial chromosome-the three-dimensional packaging of the chromosomal DNA molecule itself and its ...
sequences to coordinate the activities of proteins involved in chromosome repair and maintenance. ...
This work is supported in part by National Institutes of Health grants R01 GM51426 R24 and GM073011-04d to L.S., and NIH/NIGMS fellowship F32GM088966-3 to J.P. ...
doi:10.1111/cmi.12049
pmid:23078580
pmcid:PMC3660146
fatcat:fxzi5v2pqfbb7lcnnz5zs3o75a
Dynamic Chromosome Organization and Protein Localization Coordinate the Regulatory Circuitry that Drives the Bacterial Cell Cycle
2009
Cold Spring Harbor Symposia on Quantitative Biology
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Key insights into bacterial regulatory programs that orchestrate cell cycle progression have come from studies of Caulobacter crescentus, a bacterium that divides asymmetrically. ...
Remarkably, the transcriptional circuitry is dependent on three-dimensional dynamic deployment of key regulatory and signaling proteins. ...
Upon segregation and bipolarization of the parS/ParB/MipZ/PopZ complex, FtsZ depolymerizes at the new pole and reassembles at the site of lowest MipZ concentration in the cell, roughly the middle. ...
doi:10.1101/sqb.2009.74.005
pmid:19687139
pmcid:PMC7451405
fatcat:nbifiok43nctdd5rgrzthujv3e
Bactofilin-mediated organization of the ParABS chromosome segregation system in Myxococcus xanthus
2017
Nature Communications
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In bacteria, homologs of actin, tubulin, and intermediate filament proteins often act in concert with bacteria-specific scaffolding proteins to ensure the proper arrangement of cellular components. ...
Here, we study the three bactofilins BacNOP in the rod-shaped bacterium Myxococcus xanthus. ...
Cameron for help with the generation of demographs, Silvia G. Sierra for assistance with the flow cytometry analysis, and Patrick Schall for the construction of plasmids. ...
doi:10.1038/s41467-017-02015-z
pmid:29180656
pmcid:PMC5703909
fatcat:fqybgdg3zrbmppdq55gnygxucu
DecodingCaulobacterdevelopment
2012
FEMS Microbiology Reviews
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Caulobacter crescentus uses a multi-layered system of oscillating regulators to program different developmental fates into each daughter cell at division. ...
In this review, we outline the current state of research in the field of Caulobacter development, emphasizing new findings that elaborate how the developmental program is modulated by factors such as the ...
from the Swiss National Science Foundation (Grant # 31003A_127287), the Human Frontier Science Program (Program Grant # RGP0051/2010), the Société Académique de Genève (# 2011/70) and the University of ...
doi:10.1111/j.1574-6976.2011.00309.x
pmid:22091823
fatcat:5lmghbiq3vfaxfq2jyrvn6xk3u
Bacterial chromosome segregation by the ParABS system
2020
Open Biology
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Here, we review the various models for the formation of the ParABS complex and describe its role in segregating the origin-proximal region of the chromosome. ...
Proper chromosome segregation during cell division is essential in all domains of life. ...
In Caulobacter crescentus, Hyphomonas neptunium and Myxococcus xanthus, genes encoded in the par locus (ParABS) were found to be essential for cell viability [8] [9] [10] [11] , whereas in other bacterial ...
doi:10.1098/rsob.200097
pmid:32543349
fatcat:zg7a7oq2encorou7uspaltfhzi
Catching a Walker in the Act—DNA Partitioning by ParA Family of Proteins
2022
Frontiers in Microbiology
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Here, we provide an overview of the recent findings on the structure and function of the ParB adaptor protein and review the current models and mechanisms by which the ParA family of proteins function ...
in the partitioning of the replicated DNA into the newly born daughter cells. ...
ACKNOWLEDGMENTS The authors thank the members of the RS lab and Tirumal K. Chowdary for comments on the manuscript. ...
doi:10.3389/fmicb.2022.856547
pmid:35694299
pmcid:PMC9178275
fatcat:2wzu4q35b5fulichkukxpsxocu
RNA localization in bacteria
2014
RNA Biology
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Acknowledgments We thank members of Orna Amster-Choder's lab for fruitful discussions. ...
Funding This work was supported by the Israel Science Foundation founded by the Israel Academy of Sciences and Humanities. ...
By combining biochemical, imaging and computational methodologies, Church and coworkers derived the three-dimensional architecture of the Caulobacter crescentus chromosome and have shown it to be an ellipsoid ...
doi:10.4161/rna.36135
pmid:25482897
pmcid:PMC4615583
fatcat:obcdkot2uzejrmfwiyfju7zo6y
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