A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2018; you can also visit the original URL.
The file type is application/pdf.
Filters
Controlled Aspiration and Positioning of Biological Cells in a Micropipette
2012
IEEE Transactions on Biomedical Engineering
Preserved Fulltext
A closed-loop robust controller integrating the dynamics of cell motion was designed to accurately and efficiently position the cell to a target position inside the micropipette. ...
This paper discusses the aspiration of a single cell into a micropipette and positioning the cell accurately to a target position inside the micropipette. ...
This paper presents automation techniques (dynamic modeling and vision-based robust control) for automated single-cell aspiration into a micropipette and positioning the cell precisely inside the micropipette ...
doi:10.1109/tbme.2012.2182673
pmid:22231148
fatcat:gvxyealuubez7fxi6cjd3ujclm
Dynamical organization of the cytoskeletal cortex probed by micropipette aspiration
2010
Proceedings of the National Academy of Sciences of the United States of America
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2018; you can also visit the original URL.
The file type is application/pdf.
A cell or a GUV subjected to micropipette suction (suction pressure ΔP and pipette radius R p ) deforms until the Laplace law is satisfied both inside and outside the pipette. ...
One major parameter dictating the cell response to micropipette suction is the stationary cortex thickness, controlled by actin polymerization and depolymerization. ...
Sykes, and M. S. Turner for stimulating discussions and the group of Nancy Guillen for providing E. histolytica strains and Lifeact construct. ...
doi:10.1073/pnas.0913669107
pmid:20713731
pmcid:PMC2932608
fatcat:a4qia6mnzfeznecrb7tpdbmnze
Mechanics of membrane–cytoskeleton attachment inParamecium
2012
New Journal of Physics
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2016; you can also visit the original URL.
The file type is application/pdf.
We test this by probing the mechanics of wild-type and silenced cells by micropipette aspiration. Strikingly, we observe 8 ...
Since this structure is based on the underlying cytoskeleton, one hypothesis to explain this observation is a disruption of membrane attachment to the cytoskeleton in the absence of MKS1 that should affect ...
This work was supported by the ANR 'Programme Blanc' (ANR-08-BLAN-0012-12) and SYSCOM (ANR-08-SYSC-013-03). ...
doi:10.1088/1367-2630/14/12/125016
fatcat:l25cnc3jhbhztedlupptxqogsm
High-throughput microfluidic micropipette aspiration device to probe time-scale dependent nuclear mechanics in intact cells
[article]
2019
biorxiv/medrxiv
pre-print
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2021; you can also visit the original URL.
The file type is application/pdf.
Here, we present an easy-to-use microfluidic device that applies the well-established approach of micropipette aspiration, adapted to measure many cells in parallel. ...
In addition, we conducted experiments on cells lacking the nuclear envelope protein lamin A/C and wild-type controls, which have well-characterized nuclear mechanical properties. ...
The two pressure inlets allow precise control of the velocity of the perfusion of the cells through the devices and the pressure applied on the cells in the pockets and micropipette channels. ...
doi:10.1101/641084
fatcat:omujd3ikibbuhfrrgzmwb73wu4
Stem cell differentiation increases membrane-actin adhesion regulating cell blebability, migration and mechanics
2014
Scientific Reports
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is application/pdf.
This study examines how differentiation of human mesenchymal stem cells regulates the interaction between the cell membrane and the actin cortex controlling cell behavior. ...
Micropipette aspiration was used to measure the pressure required for membrane-cortex detachment which increased from 0.15 kPa in stem cells to 0.71 kPa following chondrogenic differentiation. ...
Charras for the kind gift of the Ezrin-T567D-GFP plasmid construct and some helpful discussion. K. S. is funded by an EPSRC PhD studentship. ...
doi:10.1038/srep07307
pmid:25471686
pmcid:PMC4255193
fatcat:kinzsqr4xzfwxej2dwtknapcaa
Passive mechanical behavior of human neutrophils: power-law fluid
1993
Biophysical Journal
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is application/pdf.
A numerical simulation was developed to interpret the experiments in terms of cell mechanical properties based on the Newtonian liquid drop model (Yeung and Evans, Biophys. J., 56:139-149, 1989). ...
The cellular mechanical properties were assessed by single cell micropipette aspiration at fixed aspiration pressures. ...
The authors would like to thank Dr. Alfred Clark, Jr., and Dr. Charles H. Packman for fruitful discussions on applied mathematics and hematology. ...
doi:10.1016/s0006-3495(93)81238-4
pmid:8298037
pmcid:PMC1225943
fatcat:rlmkgxvcqbbh5c3gmju4rlzte4
Recent Advances on the Model, Measurement Technique, and Application of Single Cell Mechanics
2020
International Journal of Molecular Sciences
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL.
The file type is application/pdf.
mechanics and discuss the latest mechanical models and experimental methods. ...
The mechanical response of cells to external stimuli and the biomechanical response inside cells are of great significance for maintaining the life activities of cells. ...
In the research process of cell mechanics, micropipette aspiration technology has been favored by many researchers. There are many new methods based on micropipette technology. ...
doi:10.3390/ijms21176248
pmid:32872378
pmcid:PMC7504142
fatcat:vhjqww6rejdyjcpx5pfasiottq
Mechanical phenotyping of K562 cells by the Micropipette Aspiration Technique allows identifying mechanical changes induced by drugs
2018
Scientific Reports
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is application/pdf.
We discuss the possible relation between the observed mechanical behavior of cells aspirated inside a micropipette and apoptosis. ...
Among these techniques there are Atomic Force Microscopy (AFM) 4,5 , Magnetic Twisting Cytometry (MTC) 6 , Micropipette Aspiration Technique (MAT) 7,8 , Particle Tracking Rheology (PTR) 9 and the Optical ...
The analysis was based on the power-law model. ...
doi:10.1038/s41598-018-19563-z
pmid:29352174
pmcid:PMC5775209
fatcat:2rgeeijtozda5l2cyyym5vjume
Mechanosensitive shivering of model tissues under controlled aspiration
2011
Proceedings of the National Academy of Sciences of the United States of America
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2018; you can also visit the original URL.
The file type is application/pdf.
Here we investigate the response of model tissues to controlled external stresses. Spherical cellular aggregates are subjected to one-dimensional stretching forces using micropipette aspiration. ...
The response to aspiration is characterized by tracking the instantaneous length of the aggregate tongue aspirated inside the micropipette, denoted by LðtÞ. ...
doi:10.1073/pnas.1105741108
pmid:21771735
pmcid:PMC3158194
fatcat:sqi66mv4cbbjrnu5e2kgjj77cm
Fluctuations of a membrane nanotube revealed by high-resolution force measurements
2016
Soft Matter
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is application/pdf.
Here we extend classical optical tweezers studies to infer membrane nanotube dynamics with high spatial and temporal resolution. ...
Then we record the position signal of a trapped bead when it is connected, or not, to a tube. ...
Acknowledgements This work was supported by the French Agence Nationale pour la Recherche (ANR), grant ANR 09BLAN0283 and ANR 12BSV5001401, and by the Fondation pour la Recherche Médicale (FRM), grant ...
doi:10.1039/c6sm02117d
pmid:27830219
fatcat:levybm2cyzccpoa3q2m2uvezdi
Biophysical Approaches for Applying and Measuring Biological Forces
2021
Advanced Science
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2022; you can also visit the original URL.
The file type is application/pdf.
Gaining a further understanding of mechanical regulation and depicting the mechanotransduction network inside cells require advanced experimental techniques and new theories. ...
The broad applications of these techniques are introduced with an emphasis on the difficulties in implementing these techniques in special biological systems. ...
This research was also supported by State Key Laboratory of Precision Measurement Technology and Instruments (Tianjin University). ...
doi:10.1002/advs.202105254
pmid:34923777
pmcid:PMC8844594
fatcat:3as3t2ph7zd2ldrlqnv672n7du
A microfluidic pipette array for mechanophenotyping of cancer cells and mechanical gating of mechanosensitive channels
2015
Lab on a Chip
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is application/pdf.
A simple microfluidic device based on a microfluidic pipette array was developed for mechanical characterization of cells. ...
We thank Johanna Heureaux, Kwun Yin Ho and Christopher Coyne for their help in the traditional micropipette aspiration experiments. The breast cell lines were kindly provided by Dr. ...
We appreciate the assistance from Victoria Murray and Johanna Heureaux in generating MscL adenoviruses and on MscL expression. ...
doi:10.1039/c4lc01218f
pmid:25361042
pmcid:PMC4256121
fatcat:y7k6qztsqzclff45gucep74kya
Quantifying single-platelet biomechanics: An outsider's guide to biophysical methods and recent advances
2020
Research and Practice in Thrombosis and Haemostasis
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL.
The file type is application/pdf.
This review focusses on principles, specific examples, and limitations of several relevant biophysical methods applied to single-platelet analysis such as micropipette aspiration, atomic force microscopy ...
Individual platelets interact and respond differentially while modulating their biomechanical behavior depending on dynamic changes that occur in surrounding microenvironments. ...
and P out are the pressures inside and outside the cell, respectively. ...
doi:10.1002/rth2.12313
pmid:32211573
pmcid:PMC7086474
fatcat:vc77viif45h3ld5mhgcduhh774
Characterizing Cell Adhesion by Using Micropipette Aspiration
2015
Biophysical Journal
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2017; you can also visit the original URL.
The file type is application/pdf.
The micropipette is positioned perpendicular to the surface of an adherent cell and a constant-rate aspiration pressure is applied. ...
Since the micropipette diameter and the aspiration pressure are our control parameters, we have direct knowledge of the aspiration force, whereas the cell behavior is monitored either in brightfield or ...
ACKNOWLEDGMENTS The authors acknowledge Julie Lafaurie-Janvore for introducing them to the micropatterning technique. ...
doi:10.1016/j.bpj.2015.06.015
pmid:26200857
pmcid:PMC4621874
fatcat:svdjuuzfrzhxfhsdtlcpsjc2vq
The Variation of Microbial Communities in a Depth Profile of an Acidic, Nutrient-Poor Boreal Bog in Southwestern Finland
2014
Open Journal of Ecology
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is application/pdf.
Micropipette aspiration measures the mechanical properties of single cells. ...
A traditional micropipette aspiration system requires a bulky infrastructure and has a low throughput and limited potential for automation. ...
We thank Johanna Heureaux, Kwun Yin Ho and Christopher Coyne for their help in the traditional micropipette aspiration experiments. The breast cell lines were kindly provided by Dr. ...
doi:10.4236/oje.2014.413071
fatcat:fdeqngphv5cz3jsuyvekleepnm
« Previous
Showing results 1 — 15 out of 737 results