@article{horvat_fulka_jankele_malik_jun_solcova_sedlacek_vlahovicek_schultz_svoboda_2018, 
  title={Role ofCnot6lin maternal mRNA turnover}, 
  DOI={10.26508/lsa.201800084}, 
  abstractNote={<jats:p>Removal of poly(A) tail is an important mechanism controlling eukaryotic mRNA turnover. The major eukaryotic deadenylase complex CCR4-NOT contains two deadenylase components, CCR4 and CAF1, for which mammalian CCR4 is encoded by<jats:italic>Cnot6</jats:italic>or<jats:italic>Cnot6l</jats:italic>paralogs. We show that<jats:italic>Cnot6l</jats:italic>apparently supplies the majority of CCR4 in the maternal CCR4-NOT in mouse, hamster, and bovine oocytes. Deletion of<jats:italic>Cnot6l</jats:italic>yielded viable mice, but<jats:italic>Cnot6l</jats:italic><jats:sup>−/−</jats:sup>females exhibited ∼40% smaller litter size. The main onset of the phenotype was post-zygotic: fertilized<jats:italic>Cnot6l</jats:italic><jats:sup>−/−</jats:sup>eggs developed slower and arrested more frequently than<jats:italic>Cnot6l</jats:italic><jats:sup>+/−</jats:sup>eggs, suggesting that maternal CNOT6L is necessary for accurate oocyte-to-embryo transition. Transcriptome analysis revealed major transcriptome changes in<jats:italic>Cnot6l</jats:italic><jats:sup>−/−</jats:sup>ovulated eggs and one-cell zygotes. In contrast, minimal transcriptome changes in preovulatory<jats:italic>Cnot6l</jats:italic><jats:sup>−/−</jats:sup>oocytes were consistent with reported<jats:italic>Cnot6l</jats:italic>mRNA dormancy. A minimal overlap between transcripts sensitive to decapping inhibition and<jats:italic>Cnot6l</jats:italic>loss suggests that decapping and CNOT6L-mediated deadenylation selectively target distinct subsets of mRNAs during oocyte-to-embryo transition in mouse.</jats:p>}, 
  publisher={Life Science Alliance, LLC}, 
  author={Horvat and Fulka and Jankele and Malik and Jun and Solcova and Sedlacek and Vlahovicek and Schultz and Svoboda}, 
  year={2018}, 
  month={Jul}
  }