Location via proxy:   [ UP ]  
[Report a bug]   [Manage cookies]                
Skip to main content
SpringerLink
Log in

An on-site immunosensor for ractopamine based on a personal glucose meter and using magnetic β-cyclodextrin-coated nanoparticles for enrichment, and an invertase-labeled nanogold probe for signal amplification

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

We report on an on-site ultrasensitive sandwich immunoassay for ractopamine (RAC) that is based on the use of a personal glucose meter. RAC is preconcentrated with the help of magnetic nanoparticles coated with β-cyclodextrin (Fe3O4@SiO2-CD), and a novel polymer tag labeled with invertase is employed to catalyze the hydrolysis of sucrose to form glucose which is detected with the glucose meter. Invertase-modified gold nanoparticles were prepared and immobilized, along with antibodies against RAC, on an Envision reagent (a polymer that contains many anti-IgG antibodies). Because of its high loading with the enzyme, the resulting copolymers strongly amplify the signal. The concentration of RAC is calculated on the basis of the linear relationship between the concentration of RAC and the amount of glucose formed. The method exhibits a detection limit of 0.34 μg kg−1 as determined for a sample of pork. This on-site testing method may also be applied to the detection of other low-molecular toxins by simply changing the antibody.

Ractopamine is preconcentrated with the help of magnetic nanoparticles coated with β-cyclodextrin (Fe3O4@SiO2-CD), and a novel polymer tag labeled with invertase is employed to recognize analyte and catalyze the hydrolysis of sucrose to form glucose which is detected with the glucose meter.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Crome PK, McKeith FK, Carr TR, Jones DJ, Mowrey DH, Cannon JE (1996) Effect of ractopamine on growth performance, carcass composition, and cutting yields of pigs slaughtered at 107 and 125 kilograms. J Anim Sci 74:709–716

    CAS  Google Scholar 

  2. Sainz RD, Kim YS, Dunshea FR, Campbell RG (1993) Effects of ractopamine in pig muscles: histology, calpains and β-adrenergic receptors. Crop Pasture Sci 44:1441–1448

    Article  CAS  Google Scholar 

  3. Sai F, Hong M, Yunfeng Z, Huijing C, Yongning W (2012) Simultaneous detection of residues of 25 β2-agonists and 23 β-blockers in animal foods by high-performance liquid chromatography coupled with linear ion trap mass spectrometry. J Agric Food Chem 60:1898–1905

    Article  CAS  Google Scholar 

  4. Gallo P, Brambilla G, Neri B, Fiori M, Testa C, Serpe L (2007) Purification of clenbuterol-like β2-agonist drugs of new generation from bovine urine and hair by α1-acid glycoprotein affinity chromatography and determination by gas chromatography–mass spectrometry. Anal Chim Acta 587:67–74

    Article  CAS  Google Scholar 

  5. He L, Su Y, Zeng Z, Liu Y, Huang X (2007) Determination of ractopamine and clenbuterol in feeds by gas chromatography–mass spectrometry. Anim Feed Sci Technol 132:316–323

    Article  CAS  Google Scholar 

  6. Du W, Fu Q, Zhao G, Huang P, Jiao Y, Wu H, Luo Z, Chang C (2013) Dummy-template molecularly imprinted solid phase extraction for selective analysis of ractopamine in pork. Food Chem 139:24–30

    Article  CAS  Google Scholar 

  7. Thompson CS, Haughey SA, Traynor IM, Fodey TL, Elliott CT, Antignac JP, Bizec LB, Crooks SR (2008) Effective monitoring for ractopamine residues in samples of animal origin by SPR biosensor and mass spectrometry. Anal Chim Acta 608:217–225

    Article  CAS  Google Scholar 

  8. He J, Fang G, Deng Q, Wang S (2011) Preparation, characterization and application of organic-inorganic hybrid ractopamine multi-template molecularly imprinted capillary monolithic column. Anal Chim Acta 692:57–62

    Article  CAS  Google Scholar 

  9. Yang X, Feng B, Yang P, Ding Y, Chen Y, Fei J (2014) Electrochemical determination of toxic ractopamine at an ordered mesoporous carbon modified electrode. Food Chem 145:619–624

    Article  CAS  Google Scholar 

  10. Lin KC, Hong CP, Chen SM (2013) Simultaneous determination for toxic ractopamine and salbutamol in pork sample using hybrid carbon nanotubes. Sensors Actuators B Chem 177:428–436

    Article  CAS  Google Scholar 

  11. Zhang MZ, Wang MZ, Chen ZL, Fang JH, Fang MM, Liu J, Yu XP (2009) Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of clenbuterol and ractopamine in swine urine. Anal Bioanal Chem 395:2591–2599

    Article  CAS  Google Scholar 

  12. Lu X, Zheng H, Li XQ, Yuan XX, Li H, Deng LG, Zhang H, Wang WZ, Yang GS, Meng M, Xi RM, Aboul-Enein HY (2012) Detection of ractopamine residues in pork by surface plasmon resonance-based biosensor inhibition immunoassay. Food Chem 130:1061–1065

    Article  CAS  Google Scholar 

  13. Xiang Y, Lu Y (2012) Portable and quantitative detection of protein biomarkers and small molecular toxins using antibodies and ubiquitous personal glucose meters. Anal Chem 84:4174–4178

    Article  CAS  Google Scholar 

  14. Yan L, Zhu Z, Zou Y, Huang Y, Liu D, Jia S, Xu D, Wu M, Zhou Y, Zhou S, Yang CJ (2013) Target-responsive sweet hydrogel with glucometer readout for portable and quantitative detection of non-glucose targets. J Am Chem Soc 135:3748–3751

    Article  CAS  Google Scholar 

  15. Gan N, Jin H, Li T, Zheng L (2011) Fe3O4/Au magnetic nanoparticle amplification strategies for ultrasensitive electrochemical immunoassay of alfa-fetoprotein. Int J Nanomedicine 6:3259–3269

    Article  CAS  Google Scholar 

  16. Kämmerer U, Kapp M, Gassel AM, Richter T, Tank C, Dietl J, Ruck P (2001) A new rapid immunohistochemical staining technique using the EnVision antibody complex. J Histochem Cytochem 49:623–630

    Article  Google Scholar 

  17. Li Y, Yuan R, Chai Y, Zhuo Y, Su H, Zhang Y (2014) Horseradish peroxidase-loaded nanospheres attached to hollow gold nanoparticles as signal enhancers in an ultrasensitive immunoassay for alpha-fetoprotein. Microchim Acta 181:679–685

    Article  CAS  Google Scholar 

  18. Zhou J, Gan N, Hu F, Li T, Zhou H, Li X, Zheng L (2013) A single antibody sandwich electrochemiluminescence immunosensor based on protein magnetic molecularly imprinted polymers mimicking capture probes. Sensors Actuators B Chem 186:300–307

    Article  CAS  Google Scholar 

  19. Guo Y, Guo S, Ren J, Zhai Y, Dong S, Wang E (2010) Cyclodextrin functionalized graphene nanosheets with high supramolecular recognition capability: synthesis and host-guest inclusion for enhanced electrochemical performance. ACS Nano 4:4001–4010

    Article  CAS  Google Scholar 

  20. Fan L, Zhang Y, Luo C, Lu F, Qiu H, Sun M (2012) Synthesis and characterization of magnetic β-cyclodextrin–chitosan nanoparticles as nano-adsorbents for removal of methyl blue. Int J Biol Macromol 50:444–450

    Article  CAS  Google Scholar 

  21. Shang K, Wang X, Sun B, Cheng Z, Ai S (2013) β-cyclodextrin-ferrocene host-guest complex multifunctional labeling triple amplification strategy for electrochemical immunoassay of subgroup J of avian leukosis viruses. Biosens Bioelectron 45:40–45

    Article  CAS  Google Scholar 

  22. Shao D, Sheng G, Chen C, Wang X, Nagatsu M (2010) Removal of polychlorinated biphenyls from aqueous solutions using β beta-cyclodextrin grafted multiwalled carbon nanotubes. Chemosphere 79:679–685

    Article  CAS  Google Scholar 

  23. Li F, Zhou R, Zhao K, Chen H, Hu Y (2011) Magnetic beads-based electrochemical immunosensor for detection of pseudorabies virus antibody in swine serum. Talanta 7:302–306

    Article  Google Scholar 

  24. Deng Y, Qi D, Deng C, Zhang X, Zhao D (2008) Superparamagnetic high-magnetization microspheres with an Fe3O4@ SiO2 core and perpendicularly aligned mesoporous SiO2 shell for removal of microcystins. J Am Chem Soc 130:28–29

    Article  CAS  Google Scholar 

  25. Shao M, Ning F, Zhao J, Wei M, Evans DG, Duan X (2012) Preparation of Fe3O4@SiO2@layered double hydroxide core-shell microspheres for magnetic separation of proteins. J Am Chem Soc 134:1071–1077

    Article  CAS  Google Scholar 

  26. Prabaharan M, Jayakumar R (2009) Chitosan-graft-beta-cyclodextrin scaffolds with controlled drug release capability for tissue engineering applications. Int J Biol Macromol 44:320–325

    Article  CAS  Google Scholar 

  27. Frens G (1973) Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nature 241:20–22

    CAS  Google Scholar 

  28. Xiong P, Gan N, Cui H, Zhou J, Cao Y, Hu F, Li T (2014) Incubation-free electrochemical immunoassay for diethylstilbestrol in milk using gold nanoparticle-antibody conjugates for signal amplification. Microchim Acta 181:453–462

    Article  CAS  Google Scholar 

  29. Liu QL, Yan XH, Yin XM, Situ B, Zhou HK, Lin L, Li B, Gan N, Zheng L (2013) Electrochemical enzyme-linked immunosorbent assay (ELISA) for α-fetoprotein based on glucose detection with multienzyme-nanoparticle amplification. Molecules 18:12675–12686

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Nos. 31070866), the Science and Technology Project of China (2012BAK08B01-2), the Natural Science Foundation of Zhejiang (LY13C200017, LY12B01005), the Natural Science Foundation of Ningbo (2013A610241, 2013A610163, 2014A610184), the Science and Technology Project of Zhejiang (2013C37033, 2013A610157, 2012C23101, and 2011C31037), and the K.C. Wong Magna Fund in Ningbo University.

Author information

Authors and Affiliations

  1. Faculty of Marine Science, Ningbo University, Ningbo, 315211, China

    Si Chen, Futao Hu & Daodong Pan

  2. The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering of Ningbo University, Ningbo 315211, People’s Republic of China

    Jiabin Zhang, Ning Gan, Tianhua Li & Yuting Cao

Authors
  1. Si Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jiabin Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ning Gan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Futao Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tianhua Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yuting Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Daodong Pan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Ning Gan or Futao Hu.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

(PDF 410 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chen, S., Zhang, J., Gan, N. et al. An on-site immunosensor for ractopamine based on a personal glucose meter and using magnetic β-cyclodextrin-coated nanoparticles for enrichment, and an invertase-labeled nanogold probe for signal amplification. Microchim Acta 182, 815–822 (2015). https://doi.org/10.1007/s00604-014-1392-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00604-014-1392-5

Keywords

Search

Navigation